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Weak cation-exchange restricted-access material for on-line purification of basic drugs in plasma.

Sato Y, Yamamoto E, Takakuwa S, Kato T, Asakawa N

Analytical Research Laboratories, Eisai Co. Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan. y12-sato@hhc.eisai.co.jp <y12-sato@hhc.eisai.co.jp>

A methylcellulose-immobilized weak cation-exchange (MC-WCX) silica-based restricted-access material (RAM) was developed. The MC-WCX consists of an MC outer surface and 2-carboxyethyl phase internal surface, allowing for direct analysis of basic drugs in plasma. The retention properties of the MC-WCX were evaluated for sulpiride, quinidine, ranitidine, and desipramine. The MC-WCX retained model drugs by cation-exchange, and retained drugs were eluted with the mobile phase containing small amount of acids or salts compared with the MC strong cation-exchanger (MC-SCX). These results indicated the ease of use of the MC-WCX solid-phase extraction (SPE) column when coupled to a reversed-phase analytical column in column-switching high-performance liquid chromatography (HPLC), and various detection principals. Further direct analysis of model drugs in plasma using the MC-WCX SPE column in a column-switching HPLC system successfully performed with sufficient recovery. It is concluded that the MC-WCX is useful for the analysis of basic drugs in plasma.

Published 21 April 2008 in J Chromatogr A, 1190(1): 8-13.
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